Journal:

Article Title: Lactoferrin acts as an alarmin to promote the recruitment and activation of antigen-presenting cells and antigen-specific immune responses

doi:

Figure Lengend Snippet: A) Monocyte migration was assayed using 48-well micro-chemotaxis chambers with the addition of monocytes (106/ml) and TLF in the upper and lower chambers, respectively. After 2 h of incubation, the membrane was scraped, fixed, and stained. Migrated monocytes were counted using Bioquant Life Science software. Results shown are the average +SD of seven experiments. *=p<0.01. B) Dose-response of monocyte migration to TLF. One experiment out of three is shown. C) Checkerboard analysis. Monocytes were allowed to migrate in the presence (+) of 1 μg/ml of TLF in the lower chamber or in both the upper and lower chambers. The results shown are the average (mean+SD) of three experiments. D) Effect of pertussis toxin (PTx). Monocytes were pretreated with 100 ng/ml PTx for 1 h at 37°C prior to utilization in the migration assay. 100ng/ml of CCL2 was used as a positive control for monocyte migration and to confirm the effect of PTx. The data show the average (mean+SD) of 4 experiments. *p<0.01; **p<0.05.

Article Snippet: In the 48-well chemotaxis assay, experiments were run in triplicates and migrated cells were fixed, stained and counted using Bioquant Life Science (Bioquant Image Analysis Corp, Nashville, TN) software.

Techniques: Migration, Chemotaxis Assay, Incubation, Staining, Software, Positive Control

Journal: JBMR Plus

Article Title: Targeting the osteoclastogenic cytokine IL-9 as a novel immunotherapeutic strategy in mitigating inflammatory bone loss in post-menopausal osteoporosis

doi: 10.1093/jbmrpl/ziae120

Figure Lengend Snippet: Anti-IL-9 immuno-therapy abrogates inflammatory bone loss under post-menopausal osteoporotic conditions: (A) Experimental plan followed for the in vivo experiment, following groups were taken including Sham, Ovx, Ovx + Isotype and Ovx + anti-IL-9 and after 45 d mice were sacrificed. (B) Micro CT data representing 3D images depicting bone microarchitecture of femur trabecular bone. (C) Plots representing the histomorphometric parameters (BMD, BV/TV, Tb.Th, Tb. No., and Tb.Sp) of femur trabecular bone. (D) Photomicrographs representing GT staining of femoral bone in different groups. (E) Static histomorphometric parameters viz. OV, OV/BV, OS, OS/BS, O.Width were quantified using osteo software (Bioquant Image Analysis, Nashville, TN). Data are expressed as mean ± SEM. Data were analyzed by unpaired Student t test and analyzed by one-way ANOVA. * p ≤ 0.05, * * p ≤ 0.01, * * * p ≤ 0.001, * * * * p ≤ 0.0001) compared with the indicated group. Abbreviations: GT, Goldner trichome; OS, osteoid surface; OS/BS, osteoid surface per bone surface; OV, osteoid volume; OV/BV, osteoid volume per bone volume; O.Width, osteoid width.

Article Snippet: Histomorphometric parameters viz. osteoid volume (OV), osteoid volume per bone volume (OV/BV), osteoid surface (OS), osteoid surface per bone surface (OS/BS), osteoid width (O.Wi) were evaluated via employing osteo software (Bioquant Image Analysis, Nashville TN).

Techniques: In Vivo, Micro-CT, Staining, Software